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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Anti-POT1 Antibody

HPA068538
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPOT1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-POT1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25913
  • Target name
    POT1
  • Target description
    protection of telomeres 1
  • Target synonyms
    CMM10, CRMCC3, GLM9, HPOT1, PFBMFT8, TPDS3, protection of telomeres protein 1, POT1-like telomere end-binding protein, protection of telomeres 1 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NUX5
  • Protein Name
    Protection of telomeres protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of POT1 using anti-POT1 antibody (PB9780). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POT1 antigen affinity purified polyclonal antibody (Catalog # PB9780) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POT1 at approximately 71KD. The expected band size for POT1 is at 71KD.
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