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Western Blot analysis of 293 cells using POT1 Polyclonal Antibody
Western Blot analysis of 293 cells using POT1 Polyclonal Antibody
Western Blot analysis of 293 cells using POT1 Polyclonal Antibody

POT1 Antibody

CSB-PA003831
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Monkey
TargetPOT1
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Overview

  • Supplier
    Cusabio
  • Product Name
    POT1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID25913
  • Target name
    POT1
  • Target description
    protection of telomeres 1
  • Target synonyms
    CMM10, CRMCC3, GLM9, HPOT1, PFBMFT8, TPDS3, protection of telomeres protein 1, POT1-like telomere end-binding protein, protection of telomeres 1 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NUX5
  • Protein Name
    Protection of telomeres protein 1
  • Reactivity
    Human, Monkey
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of POT1 using anti-POT1 antibody (PB9780). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POT1 antigen affinity purified polyclonal antibody (Catalog # PB9780) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for POT1 at approximately 71KD. The expected band size for POT1 is at 71KD.
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