More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-PPIE Antibody

Anti-PPIE Antibody

144-07061
RayBiotech
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetPPIE
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-PPIE Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10450
  • Target name
    PPIE
  • Target description
    peptidylprolyl isomerase E
  • Target synonyms
    cyclophilin-33; CYP33; CYP-33; peptidyl-prolyl cis-trans isomerase E; peptidylprolyl isomerase E (cyclophilin E); PPIase E; rotamase E
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UNP9
  • Protein Name
    Peptidyl-prolyl cis-trans isomerase E
  • Scientific Description
    PPIE Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Biomatik
PPIE Polyclonal AntibodyCAC13541
ApplicationsELISA, ImmunoHistoChemistry
TargetPPIE
  • SizePrice
Product group Antibodies
Assay Genie
PPIE AntibodyCAB7061
TargetPPIE
  • SizePrice
IHC image of CSB-PA890944LA01HU diluted at 1:300 and staining in paraffin-embedded human adrenal gland tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
Cusabio
PPIE AntibodyCSB-PA890944LA01HU
ApplicationsELISA, ImmunoHistoChemistry
TargetPPIE
  • SizePrice
Figure 1. Western blot analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (A08021-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclophilin E/PPIE antigen affinity purified polyclonal antibody (Catalog # A08021-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclophilin E/PPIE at approximately 35KD. The expected band size for Cyclophilin E/PPIE is at 35KD.
Product group Antibodies
Boster Bio
Anti-Cyclophilin E/PPIE Antibody Picoband(r)A08021-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetPPIE
  • SizePrice
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
Product group Antibodies
Atlas Antibodies
Anti-PPIE AntibodyHPA019357
ApplicationsImmunoCytoChemistry
TargetPPIE
  • SizePrice
Product group Antibodies
GeneTex
Cyclophilin E antibodyGTX103662
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetPPIE
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cyclophilin E Polyclonal Antibody, Unconjugated (bs-14138R) at 1:200 overnight at 4°C, DAB staining.
Product group Antibodies
Bioss Antibodies
Cyclophilin E Polyclonal AntibodyBS-14138R
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetPPIE
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PPIE / Cyclophilin E AntibodyLS-C349020
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
TargetPPIE
  • SizePrice
Product group Antibodies
Antibodies.com
Anti-PPIE AntibodyA31845
ApplicationsWestern Blot, ImmunoHistoChemistry
  • SizePrice