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Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cyclophilin E Polyclonal Antibody, Unconjugated (bs-14138R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cyclophilin E Polyclonal Antibody, Unconjugated (bs-14138R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Cyclophilin E Polyclonal Antibody, Unconjugated (bs-14138R) at 1:200 overnight at 4°C, DAB staining.

Cyclophilin E Polyclonal Antibody

BS-14138R
Bioss
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPPIE
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Overview

  • Supplier
    Bioss
  • Product Name
    Cyclophilin E Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF()
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID10450
  • Target name
    PPIE
  • Target description
    peptidylprolyl isomerase E
  • Target synonyms
    CYP-33, CYP33, CypE, peptidyl-prolyl cis-trans isomerase E, PPIase E, cyclophilin-33, peptidylprolyl isomerase E (cyclophilin E), rotamase E
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UNP9
  • Protein Name
    Peptidyl-prolyl cis-trans isomerase E
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Cyclophilin E/PPIE using anti-Cyclophilin E/PPIE antibody (A08021-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclophilin E/PPIE antigen affinity purified polyclonal antibody (Catalog # A08021-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclophilin E/PPIE at approximately 35KD. The expected band size for Cyclophilin E/PPIE is at 35KD.
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