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Immunohistochemical staining of human tonsil shows strong nuclear positivity in germinal center cells.
Immunohistochemical staining of human tonsil shows strong nuclear positivity in germinal center cells.
Immunohistochemical staining of human tonsil shows strong nuclear positivity in germinal center cells.

Anti-PPIL2 Antibody

HPA035344
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetPPIL2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PPIL2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23759
  • Target name
    PPIL2
  • Target description
    peptidylprolyl isomerase like 2
  • Target synonyms
    CYC4; cyclophilin, 60kDa; cyclophilin-60; cyclophilin-like protein CyP-60; CYP60; Cyp-60; hCyP-60; peptidylprolyl cis-trans isomerase; peptidyl-prolyl cis-trans isomerase-like 2; peptidylprolyl isomerase (cyclophilin)-like 2; PPIase; probable inactive peptidyl-prolyl cis-trans isomerase-like 2; RING-type E3 ubiquitin transferase isomerase-like 2; RING-type E3 ubiquitin-protein ligase PPIL2; rotamase PPIL2; U-box domain containing 7; UBOX7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13356
  • Protein Name
    RING-type E3 ubiquitin-protein ligase PPIL2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PPIL2 using anti-PPIL2 antibody (A11451-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPIL2 antigen affinity purified polyclonal antibody (Catalog # A11451-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPIL2 at approximately 59 kDa. The expected band size for PPIL2 is at 59 kDa.
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