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Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA621661ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA621661ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA621661ESR1HU at dilution of 1:100

PPIL2 Antibody

CSB-PA621661ESR1HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPPIL2
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Overview

  • Supplier
    Cusabio
  • Product Name
    PPIL2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23759
  • Target name
    PPIL2
  • Target description
    peptidylprolyl isomerase like 2
  • Target synonyms
    CYC4; cyclophilin, 60kDa; cyclophilin-60; cyclophilin-like protein CyP-60; CYP60; Cyp-60; hCyP-60; peptidylprolyl cis-trans isomerase; peptidyl-prolyl cis-trans isomerase-like 2; peptidylprolyl isomerase (cyclophilin)-like 2; PPIase; probable inactive peptidyl-prolyl cis-trans isomerase-like 2; RING-type E3 ubiquitin transferase isomerase-like 2; RING-type E3 ubiquitin-protein ligase PPIL2; rotamase PPIL2; U-box domain containing 7; UBOX7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13356
  • Protein Name
    RING-type E3 ubiquitin-protein ligase PPIL2
  • Scientific Description
    May catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides thereby assisting the folding of proteins (By similarity). May also function as a chaperone, playing a role in transport to the cell membrane of BSG for instance (PubMed:15946952). May also have a protein ubiquitin ligase activity acting as an E3 ubiquitin protein ligase or as an ubiquitin-ubiquitin ligase promoting elongation of ubiquitin chains on substrates. By mediating Lys-48-linked polyubiquitination of proteins could target them for proteasomal degradation (PubMed:11435423).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PPIL2 using anti-PPIL2 antibody (A11451-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPIL2 antigen affinity purified polyclonal antibody (Catalog # A11451-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPIL2 at approximately 59 kDa. The expected band size for PPIL2 is at 59 kDa.
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