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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells and neuropil.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells and neuropil.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells and neuropil.

Anti-PPP2CA Antibody

HPA043236
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPPP2CA
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PPP2CA
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5515
  • Target name
    PPP2CA
  • Target description
    protein phosphatase 2 catalytic subunit alpha
  • Target synonyms
    HJS3, NEDLBA, PP2Ac, PP2CA, PP2Calpha, RP-C, serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PP2A-alpha, protein phosphatase 2, catalytic subunit, alpha isozyme, replication protein C
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP67775
  • Protein Name
    Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PPP2CA using anti-PPP2CA antibody (PB9347). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse lung tissue lysates, Lane 9: mouse liver tissue lysates, Lane 10: mouse brain tissue lysates, Lane 11: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP2CA antigen affinity purified polyclonal antibody (Catalog # PB9347) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP2CA at approximately 34, 36 kDa. The expected band size for PPP2CA is at 36 kDa.
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