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Immunohistochemical staining of human hippocampus shows moderate nuclear positivity in neurons.
Immunohistochemical staining of human hippocampus shows moderate nuclear positivity in neurons.
Immunohistochemical staining of human hippocampus shows moderate nuclear positivity in neurons.

Anti-PPP2R5B Antibody

HPA036607
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPPP2R5B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PPP2R5B Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5526
  • Target name
    PPP2R5B
  • Target description
    protein phosphatase 2 regulatory subunit B'beta
  • Target synonyms
    B56B, B56beta, PR61B, serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform, protein phosphatase 2 regulatory subunit B', beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15173
  • Protein Name
    Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP2R5B antibody [C3], C-term (GTX106400) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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Figure 1. Western blot analysis of PPP2R5B using anti-PPP2R5B antibody (A09894-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP2R5B antigen affinity purified polyclonal antibody (Catalog # A09894-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP2R5B at approximately 65 kDa. The expected band size for PPP2R5B is at 57 kDa.
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