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ProductsBack/PPP2R5B antibody [C3], C-term
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP2R5B antibody [C3], C-term (GTX106400) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP2R5B antibody [C3], C-term (GTX106400) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PPP2R5B antibody [C3], C-term (GTX106400) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

PPP2R5B antibody [C3], C-term

GTX106400
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetPPP2R5B
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Overview

  • Supplier
    GeneTex
  • Product Name
    PPP2R5B antibody [C3], C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.55 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5526
  • Target name
    PPP2R5B
  • Target description
    protein phosphatase 2 regulatory subunit B'beta
  • Target synonyms
    B56B, B56beta, PR61B, serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform, protein phosphatase 2 regulatory subunit B', beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15173
  • Protein Name
    Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform
  • Scientific Description
    The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes a beta isoform of the regulatory subunit B56 subfamily. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PPP2R5B using anti-PPP2R5B antibody (A09894-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human SIHA whole cell lysates, Lane 3: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP2R5B antigen affinity purified polyclonal antibody (Catalog # A09894-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP2R5B at approximately 65 kDa. The expected band size for PPP2R5B is at 57 kDa.
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