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ProductsBack/Anti-PPP3CB Mouse Monoclonal Antibody [Clone ID: OTI2E4]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP3CB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP3CB (1:2000).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP3CB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP3CB (1:2000).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPP3CB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPP3CB (1:2000).

Anti-PPP3CB Mouse Monoclonal Antibody [Clone ID: OTI2E4]

M07303
Boster Bio
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPPP3CB
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-PPP3CB Mouse Monoclonal Antibody [Clone ID: OTI2E4]
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI2E4
  • Concentration
    1 mg/ml
  • Gene ID5532
  • Target name
    PPP3CB
  • Target description
    protein phosphatase 3 catalytic subunit beta
  • Target synonyms
    CALNA2, CALNB, CNA2, PP2Bbeta, serine/threonine-protein phosphatase 2B catalytic subunit beta isoform, CAM-PRP catalytic subunit, CNA beta, calcineurin A beta, calcineurin A2, protein phosphatase 3, catalytic subunit, beta isozyme, protein phosphatase from PCR fragment H32
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDP16298
  • Protein Name
    Serine/threonine-protein phosphatase 2B catalytic subunit beta isoform
  • Scientific Description
    Boster Bio PPP3CB mouse monoclonal antibody,clone OTI2E4. Catalog# M07303. Tested in WB. This antibody reacts with Human, Mouse, Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of PPP3CB using anti-PPP3CB antibody (A07303-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat heart tissue lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse brain tissue lysates, Lane 7: mouse heart tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP3CB antigen affinity purified polyclonal antibody (Catalog # A07303-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP3CB at approximately 59 kDa. The expected band size for PPP3CB is at 59 kDa.
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