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Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.

Anti-PPP6R3 Antibody

HPA038468
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPPP6R3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PPP6R3 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55291
  • Target name
    PPP6R3
  • Target description
    protein phosphatase 6 regulatory subunit 3
  • Target synonyms
    C11orf23, PP6R3, SAP190, SAPL, SAPLa, SAPS3, serine/threonine-protein phosphatase 6 regulatory subunit 3, SAPS domain family, member 3, sporulation-induced transcript 4-associated protein SAPL
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5H9R7
  • Protein Name
    Serine/threonine-protein phosphatase 6 regulatory subunit 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SAPS3/PPP6R3 using anti-SAPS3/PPP6R3 antibody (A10833-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human U-87MG whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat NRK whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SAPS3/PPP6R3 antigen affinity purified polyclonal antibody (Catalog # A10833-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SAPS3/PPP6R3 at approximately 144 kDa. The expected band size for SAPS3/PPP6R3 is at 95 kDa.
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