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Immunohistochemical staining of human thyroid gland shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human thyroid gland shows moderate cytoplasmic positivity in glandular cells.

Anti-PRAG1 Antibody

HPA012066
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPRAG1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PRAG1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID157285
  • Target name
    PRAG1
  • Target description
    PEAK1 related, kinase-activating pseudokinase 1
  • Target synonyms
    NACK, PEAK2, PRAGMIN, SGK223, inactive tyrosine-protein kinase PRAG1, PEAK family member 2, Pragmin, RND2 effector protein, homolog of rat pragma of Rnd2, sugen kinase 223, tyrosine-protein kinase PRAG1, tyrosine-protein kinase SgK223
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86YV5
  • Protein Name
    Inactive tyrosine-protein kinase PRAG1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PRAG1 using anti-PRAG1 antibody (A32113). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRAG1 antigen affinity purified polyclonal antibody (Catalog # A32113) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRAG1 at approximately 200 kDa. The expected band size for PRAG1 is at 150 kDa.
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