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Western Blot analysis of A549 cells using Phospho-Pragmin (Y413) Polyclonal Antibody
Western Blot analysis of A549 cells using Phospho-Pragmin (Y413) Polyclonal Antibody
Western Blot analysis of A549 cells using Phospho-Pragmin (Y413) Polyclonal Antibody

Phospho-SGK223 (Y413) Antibody

CSB-PA007578
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPRAG1
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Overview

  • Supplier
    Cusabio
  • Product Name
    Phospho-SGK223 (Y413) Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID157285
  • Target name
    PRAG1
  • Target description
    PEAK1 related, kinase-activating pseudokinase 1
  • Target synonyms
    NACK, PEAK2, PRAGMIN, SGK223, inactive tyrosine-protein kinase PRAG1, PEAK family member 2, Pragmin, RND2 effector protein, homolog of rat pragma of Rnd2, sugen kinase 223, tyrosine-protein kinase PRAG1, tyrosine-protein kinase SgK223
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86YV5
  • Protein Name
    Inactive tyrosine-protein kinase PRAG1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PRAG1 using anti-PRAG1 antibody (A32113). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRAG1 antigen affinity purified polyclonal antibody (Catalog # A32113) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRAG1 at approximately 200 kDa. The expected band size for PRAG1 is at 150 kDa.
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