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Anti-PRDX2 [SAIC-34B-124]

Ab00327-1.1
Absolute Antibody
ApplicationsMass Spectrometry, Western Blot, ELISA
Product group Antibodies
TargetPRDX2
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-PRDX2 [SAIC-34B-124]
  • Delivery Days Customer
    7
  • Application Supplier Note
    Original data characterizing this antibody may be found here .
  • Applications
    Mass Spectrometry, Western Blot, ELISA
  • Applications Supplier
    ELISA; WB; immunoMRM
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SAIC-34B-124
  • Gene ID7001
  • Target name
    PRDX2
  • Target description
    peroxiredoxin 2
  • Target synonyms
    HEL-S-2a, NKEF-B, NKEFB, PRP, PRX2, PRXII, PTX1, TDPX1, TPX1, TSA, peroxiredoxin-2, epididymis secretory sperm binding protein Li 2a, natural killer cell-enhancing factor B, thiol-specific antioxidant 1, thioredoxin peroxidase 1, thioredoxin-dependent peroxide reductase 1, thioredoxin-dependent peroxiredoxin 2, torin
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP32119
  • Protein Name
    Peroxiredoxin-2
  • Scientific Description
    This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Peptide "LSEDYGVLK", derived from the cellular redox regulator Peroxiredoxin-2, conjugated to KLH.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Lane 1: SW480 cell lysates; Lane 2: 22Rv1 cell lysates; Lane 3: HepG2 cell lysates; Lane 4: HL-60 cell lysates; Lane 5: K562 cell lysates; Lane 6: Mouse brain lysates; Lane 7: Mouse kidney lysates probed with PRDX2 Polyclonal Antibody, Unconjugated (bs-55172R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of Peroxiredoxin 2/PRDX2 using anti-Peroxiredoxin 2/PRDX2 antibody (A01982-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Peroxiredoxin 2/PRDX2 antigen affinity purified polyclonal antibody (Catalog # A01982-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Peroxiredoxin 2/PRDX2 at approximately 22 kDa. The expected band size for Peroxiredoxin 2/PRDX2 is at 22 kDa.
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