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PRX II antibody

GTX100747
GeneTex
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPRDX2
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Overview

  • Supplier
    GeneTex
  • Product Name
    PRX II antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.44 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7001
  • Target name
    PRDX2
  • Target description
    peroxiredoxin 2
  • Target synonyms
    epididymis secretory sperm binding protein Li 2a; HEL-S-2a; natural killer cell-enhancing factor B; NKEFB; NKEF-B; peroxiredoxin-2; PRP; PRX2; PRXII; PTX1; TDPX1; thiol-specific antioxidant 1; thioredoxin peroxidase 1; thioredoxin-dependent peroxide reductase 1; thioredoxin-dependent peroxiredoxin 2; torin; TPX1; TSA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP32119
  • Protein Name
    Peroxiredoxin-2
  • Scientific Description
    This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. The crystal structure of this protein has been resolved to 2.7 angstroms. Transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Proteomic analysis of rhein-induced cyt: ER stress mediates cell death in breast cancer cells. Huang HJ et al., 2014 Dec, Mol Biosyst
    Read more
  • Proteomic identification of VEGF-dependent protein enrichment to membrane caveolar-raft microdomains in endothelial progenitor cells. Chilla A et al., 2013 Jul, Mol Cell Proteomics
    Read more
  • Proteomic analysis of proteins responsible for the development of doxorubicin resistance in human uterine cancer cells. Lin ST et al., 2012 Oct 22, J Proteomics
    Read more

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Lane 1: SW480 cell lysates; Lane 2: 22Rv1 cell lysates; Lane 3: HepG2 cell lysates; Lane 4: HL-60 cell lysates; Lane 5: K562 cell lysates; Lane 6: Mouse brain lysates; Lane 7: Mouse kidney lysates probed with PRDX2 Polyclonal Antibody, Unconjugated (bs-55172R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of Peroxiredoxin 2/PRDX2 using anti-Peroxiredoxin 2/PRDX2 antibody (A01982-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Peroxiredoxin 2/PRDX2 antigen affinity purified polyclonal antibody (Catalog # A01982-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Peroxiredoxin 2/PRDX2 at approximately 22 kDa. The expected band size for Peroxiredoxin 2/PRDX2 is at 22 kDa.
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