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Immunohistochemical staining of human stomach shows strong nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows strong nuclear and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows strong nuclear and cytoplasmic positivity in glandular cells.

Anti-PRDX6 Antibody

HPA006983
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPRDX6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PRDX6 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9588
  • Target name
    PRDX6
  • Target description
    peroxiredoxin 6
  • Target synonyms
    1-Cys, AOP2, HEL-S-128m, LPCAT-5, NSGPx, PRX, aiPLA2, p29, peroxiredoxin-6, 1-Cys PRX, 1-Cys peroxiredoxin, 24 kDa protein, LPC acyltransferase 5, acidic calcium-independent phospholipase A2, antioxidant protein 2, epididymis secretory sperm binding protein Li 128m, glutathione-dependent peroxiredoxin, liver 2D page spot 40, lyso-PC acyltransferase 5, lysophosphatidylcholine acyltransferase 5, non-selenium glutathione peroxidase, red blood cells page spot 12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30041
  • Protein Name
    Peroxiredoxin-6
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PRDX6 using anti-PRDX6 antibody (PB9350). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDX6 antigen affinity purified polyclonal antibody (Catalog # PB9350) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRDX6 at approximately 25 kDa. The expected band size for PRDX6 is at 25 kDa.
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