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Peroxiredoxin 6 antibody

GTX115262
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetPRDX6
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Overview

  • Supplier
    GeneTex
  • Product Name
    Peroxiredoxin 6 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.89 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9588
  • Target name
    PRDX6
  • Target description
    peroxiredoxin 6
  • Target synonyms
    1-Cys, AOP2, HEL-S-128m, LPCAT-5, NSGPx, PRX, aiPLA2, p29, peroxiredoxin-6, 1-Cys PRX, 1-Cys peroxiredoxin, 24 kDa protein, LPC acyltransferase 5, acidic calcium-independent phospholipase A2, antioxidant protein 2, epididymis secretory sperm binding protein Li 128m, glutathione-dependent peroxiredoxin, liver 2D page spot 40, lyso-PC acyltransferase 5, lysophosphatidylcholine acyltransferase 5, non-selenium glutathione peroxidase, red blood cells page spot 12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30041
  • Protein Name
    Peroxiredoxin-6
  • Scientific Description
    The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Chang CH, Lo WY, Lee TH. The Antioxidant Peroxiredoxin 6 (Prdx6) Exhibits Different Profiles in the Livers of Seawater- and Fresh Water-Acclimated Milkfish, Chanos chanos, upon Hypothermal Challenge. Front Physiol. 2016,7:580.
    Read this paper
  • Chen JY, Hu RY, Chou HC. Quercetin-induced cardioprotection against doxorubicin cytotoxicity. J Biomed Sci. 2013,20(1):95. doi: 10.1186/1423-0127-20-95
    Read this paper
  • Chen JY, Chou HC, Chen YH, et al. High glucose-induced proteome alterations in hepatocytes and its possible relevance to diabetic liver disease. J Nutr Biochem. 2013,24(11):1889-910. doi: 10.1016/j.jnutbio.2013.05.006
    Read this paper
  • Liu Z, Li Q, Huang J, et al. Proteomic analysis of the inhibitory effect of epigallocatechin gallate on lipid accumulation in human HepG2 cells. Proteome Sci. 2013,11(1):32. doi: 10.1186/1477-5956-11-32
    Read this paper
  • Li JM, Chou HC, Wang SH, et al. Hyaluronic acid-dependent protection against UVB-damaged human corneal cells. Environ Mol Mutagen. 2013,54(6):429-49. doi: 10.1002/em.21794
    Read this paper
  • Chillà A, Magherini F, Margheri F, et al. Proteomic identification of VEGF-dependent protein enrichment to membrane caveolar-raft microdomains in endothelial progenitor cells. Mol Cell Proteomics. 2013,12(7):1926-38. doi: 10.1074/mcp.M112.024638
    Read this paper

Datasheet

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Figure 1. Western blot analysis of PRDX6 using anti-PRDX6 antibody (PB9350). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human 293T whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse lung tissue lysates, Lane 8: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDX6 antigen affinity purified polyclonal antibody (Catalog # PB9350) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRDX6 at approximately 25 kDa. The expected band size for PRDX6 is at 25 kDa.
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