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Immunofluorescent staining of human cell line U2OS shows localization to endoplasmic reticulum.
Immunofluorescent staining of human cell line U2OS shows localization to endoplasmic reticulum.
Immunofluorescent staining of human cell line U2OS shows localization to endoplasmic reticulum.

Anti-PRELP Antibody

HPA059225
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPRELP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PRELP Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5549
  • Target name
    PRELP
  • Target description
    proline and arginine rich end leucine rich repeat protein
  • Target synonyms
    MST161, MSTP161, SLRR2A, prolargin, 55 kDa leucine-rich repeat protein of articular cartilage, prolargin proteoglycan, proline-arginine-rich end leucine-rich repeat protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP51888
  • Protein Name
    Prolargin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PRELP using anti-PRELP antibody (A07640-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRELP antigen affinity purified polyclonal antibody (Catalog # A07640-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRELP at approximately 65 kDa. The expected band size for PRELP is at 44,65 kDa.
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