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Immunofluorescent staining of human cell line HEK 293 shows localization to endoplasmic reticulum.
Immunofluorescent staining of human cell line HEK 293 shows localization to endoplasmic reticulum.
Immunofluorescent staining of human cell line HEK 293 shows localization to endoplasmic reticulum.

Anti-PREX2 Antibody

HPA075956
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPREX2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PREX2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80243
  • Target name
    PREX2
  • Target description
    phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 2
  • Target synonyms
    DEP.2, DEPDC2, P-REX2, PPP1R129, phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein, DEP domain-containing protein 2, PtdIns(3,4,5)-dependent Rac exchanger 2, protein phosphatase 1, regulatory subunit 129
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ70Z35
  • Protein Name
    Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PREX2 using anti-PREX2 antibody (A06715-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse skeletal muscle lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PREX2 antigen affinity purified polyclonal antibody (Catalog # A06715-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PREX2 at approximately 183 kDa. The expected band size for PREX2 is at 183 kDa.
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