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Immunofluorescent staining of human cell line A549 shows localization to microtubule organizing center.
Immunofluorescent staining of human cell line A549 shows localization to microtubule organizing center.
Immunofluorescent staining of human cell line A549 shows localization to microtubule organizing center.

Anti-PRKCQ Antibody

HPA065279
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetPRKCQ
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PRKCQ
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5588
  • Target name
    PRKCQ
  • Target description
    protein kinase C theta
  • Target synonyms
    nPKC-theta; PRKCT; protein kinase C theta type
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ04759
  • Protein Name
    Protein kinase C theta type
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PKC Theta/PRKCQ using anti-PKC Theta/PRKCQ antibody (A01293-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human blood lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC Theta/PRKCQ antigen affinity purified polyclonal antibody (Catalog # A01293-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKC Theta/PRKCQ at approximately 78KD. The expected band size for PKC Theta/PRKCQ is at 78KD.
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