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PRKCQ Antibody

CAB5834
Assay Genie
TargetPRKCQ
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    PRKCQ Antibody
  • Delivery Days Customer
    9
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID5588
  • Target name
    PRKCQ
  • Target description
    protein kinase C theta
  • Target synonyms
    PRKCT, nPKC-theta, protein kinase C theta type
  • Host
    Rabbit
  • Protein IDQ04759
  • Protein Name
    Protein kinase C theta type
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human,Mouse,Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Lane 1: Mouse Cerebrum lysates; Lane 2: Mouse Cerebellum lysates probed with PRKCQ(Thr538) Polyclonal Antibody, Unconjugated (bs-5585R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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References
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Figure 1. Western blot analysis of PKC Theta/PRKCQ using anti-PKC Theta/PRKCQ antibody (A01293-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human blood lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKC Theta/PRKCQ antigen affinity purified polyclonal antibody (Catalog # A01293-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKC Theta/PRKCQ at approximately 78KD. The expected band size for PKC Theta/PRKCQ is at 78KD.
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