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Immunohistochemical staining of human placenta shows strong cytoplasmic and membranous positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic and membranous positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic and membranous positivity in trophoblastic cells.

Anti-PRKG1 Antibody

HPA007699
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetPRKG1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PRKG1
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5592
  • Target name
    PRKG1
  • Target description
    protein kinase cGMP-dependent 1
  • Target synonyms
    AAT8; cGK; cGK 1; cGK1; cGKI; cGKI-alpha; cGKI-BETA; cGMP-dependent protein kinase 1; PKG; PKG1; PRKG1B; PRKGR1B; protein kinase, cGMP-dependent, regulatory, type I, beta; protein kinase, cGMP-dependent, type I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13976
  • Protein Name
    cGMP-dependent protein kinase 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CGKI/PRKG1 using anti-CGKI/PRKG1 antibody (A01708-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CGKI/PRKG1 antigen affinity purified polyclonal antibody (Catalog # A01708-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CGKI/PRKG1 at approximately 78KD. The expected band size for CGKI/PRKG1 is at 78KD.
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