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cGKI Polyclonal Antibody

BS-6705R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityBovine, Canine, Chicken, Human, Mouse, Porcine, Rat
TargetPRKG1
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Overview

  • Supplier
    Bioss
  • Product Name
    cGKI Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(WB=1:500-2000), ICC/IF(ICC/IF=1:100)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID5592
  • Target name
    PRKG1
  • Target description
    protein kinase cGMP-dependent 1
  • Target synonyms
    AAT8, PKG, PKG1, PRKG1B, PRKGR1B, cGK, cGK 1, cGK1, cGKI, cGKI-BETA, cGKI-alpha, cGMP-dependent protein kinase 1, protein kinase, cGMP-dependent, regulatory, type I, beta, protein kinase, cGMP-dependent, type I
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Bovine, Canine, Chicken, Human, Mouse, Porcine, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Huan X, Oumei C, Hongmei Q, et al. PDE9 inhibition promotes proliferation of neural stem cells via cGMP-PKG pathway following oxygen-glucose deprivation/reoxygenation injury in vitro. Neurochem Int. 2020,133:104630. doi: 10.1016/j.neuint.2019.104630
    Read this paper

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Figure 1. Western blot analysis of CGKI/PRKG1 using anti-CGKI/PRKG1 antibody (A01708-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat lung tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CGKI/PRKG1 antigen affinity purified polyclonal antibody (Catalog # A01708-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CGKI/PRKG1 at approximately 78KD. The expected band size for CGKI/PRKG1 is at 78KD.
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