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Anti-properdin [3A3E1]

AB02512-1.1
Absolute Antibody
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCFP
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-properdin [3A3E1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    The specificity of the original format of the antibody was confirmed by ELISA analysis. The antibody detected only non-reduced properdin as evaluated by western blot analysis. The antibody inhibited AP-mediated hemolysis of rabbit erythrocytes. The ability of the antibody to inhibit the binding of properdin to C3b was tested by flow cytometry. The antibody completely impaired the binding of properdin to C3b-coated erythrocytes (ESC3b) (Chen et al. 2020; PMID: 32793201). Further, the antibody inhibited PGA formation relative to TRAP alone (Blatt et al., 2016; PMID: 27183616).
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3A3E1
  • Gene ID5199
  • Target name
    CFP
  • Target description
    complement factor properdin
  • Target synonyms
    BFD; complement factor P; PFC; PFD; properdin; properdin P factor, complement
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP27918
  • Protein Name
    Properdin
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CFP using anti-CFP antibody (A00852-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-937 whole cell lysate, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CFP antigen affinity purified polyclonal antibody (Catalog # A00852-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CFP at approximately 51KD. The expected band size for CFP is at 51KD.
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