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Western blot analysis of PROX1 expression in HepG2 cell lysate.
Western blot analysis of PROX1 expression in HepG2 cell lysate.
Western blot analysis of PROX1 expression in HepG2 cell lysate.

Anti-PROX1 Rabbit Monoclonal Antibody

M01985-1
Boster Bio
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetPROX1
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-PROX1 Rabbit Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AOGA-16
  • Gene ID5629
  • Target name
    PROX1
  • Target description
    prospero homeobox 1
  • Target synonyms
    homeobox prospero-like protein PROX1; prospero homeobox protein 1; prospero-related homeobox 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92786
  • Protein Name
    Prospero homeobox protein 1
  • Scientific Description
    Boster Bio Anti-PROX1 Rabbit Monoclonal Antibody catalog # M01985-1. Tested in IHC, WB application. This antibody reacts with Human.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PROX1 using anti-PROX1 antibody (A01985-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PROX1 antigen affinity purified polyclonal antibody (Catalog # A01985-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PROX1 at approximately 90 kDa. The expected band size for PROX1 is at 83 kDa.
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