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Anti-PRPF4 Antibody

Anti-PRPF4 Antibody

A16691
Antibodies.com
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat

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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-PRPF4 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to PRPF4.
  • Reactivity
    Human, Mouse, Rat
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PRPF4 using anti-PRPF4 antibody (A08225-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF4 antigen affinity purified polyclonal antibody (Catalog # A08225-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRPF4 at approximately 55 kDa. The expected band size for PRPF4 is at 58kDa.
Product group Antibodies
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Anti-PRPF4 Antibody Picoband(r)A08225-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPRPF4
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Product group Antibodies
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ReactivityHuman, Mouse, Rat
TargetPRPF4
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Product group Antibodies
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Pre-MRNA Processing Factor 4 Recombinant Antbody Kit (KD-Validated)BSM-90451R
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry
ReactivityHuman, Mouse, Rat
TargetPRPF4
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Western blot All lanes: PRPF4 antibody at 2.87microg/ml Lane 1: Jurkat whole cell lysate Lane 2: Hela whole cell lysate Lane 3: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 59 kDa Observed band size: 59 kDa
Product group Antibodies
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Immunohistochemical staining of human cerebral cortex, endometrium, testis and tonsil using Anti-PRPF4 antibody HPA021794 (A) shows similar protein distribution across tissues to independent antibody HPA022248 (B).
Product group Antibodies
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Sample (30 ug of whole cell lysate) A: HeLa 7.5% SDS PAGE GTX120537 diluted at 1:1000
Product group Antibodies
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PRPF4 antibody [N3C3]GTX120537
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ReactivityHuman
TargetPRPF4
  • SizePrice