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Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.
Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic positivity in neuropil.

Anti-PRRT1 Antibody

HPA055149
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPRRT1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PRRT1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80863
  • Target name
    PRRT1
  • Target description
    proline rich transmembrane protein 1
  • Target synonyms
    C6orf31, DSPD1, IFITMD7, NG5, SynDIG4, proline-rich transmembrane protein 1, dispanin subfamily D member 1, interferon induced transmembrane protein domain containing 7, synapse differentiation-induced protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99946
  • Protein Name
    Proline-rich transmembrane protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of SynDIG4/PRRT1 using anti-SynDIG4/PRRT1 antibody (A13564-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SynDIG4/PRRT1 antigen affinity purified polyclonal antibody (Catalog # A13564-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SynDIG4/PRRT1 at approximately 36 kDa. The expected band size for SynDIG4/PRRT1 is at 36 kDa.
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Immunohistochemical staining of human hippocampus shows strong cytoplasmic and nuclear positivity in neuronal cells.
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