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PRRT1 Antibody

LS-C754285
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetPRRT1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PRRT1 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    ELISA, IF, WB Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID80863
  • Target name
    PRRT1
  • Target description
    proline rich transmembrane protein 1
  • Target synonyms
    C6orf31, DSPD1, IFITMD7, NG5, SynDIG4, proline-rich transmembrane protein 1, dispanin subfamily D member 1, interferon induced transmembrane protein domain containing 7, synapse differentiation-induced protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SynDIG4/PRRT1 using anti-SynDIG4/PRRT1 antibody (A13564-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SynDIG4/PRRT1 antigen affinity purified polyclonal antibody (Catalog # A13564-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SynDIG4/PRRT1 at approximately 36 kDa. The expected band size for SynDIG4/PRRT1 is at 36 kDa.
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