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Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subset of non germinal center cells.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subset of non germinal center cells.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subset of non germinal center cells.

Anti-PSD Antibody

HPA059237
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPSD
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PSD Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5662
  • Target name
    PSD
  • Target description
    pleckstrin and Sec7 domain containing
  • Target synonyms
    EFA6, EFA6A, PSD1, TYL, PH and SEC7 domain-containing protein 1, exchange factor for ADP-ribosylation factor guanine nucleotide factor 6, exchange factor for ARF6 A, pleckstrin homology and SEC7 domain-containing protein 1, pleckstrin-Sec7 domains-containing
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDA5PKW4
  • Protein Name
    PH and SEC7 domain-containing protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of PSD using anti-PSD antibody (A01427). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSD antigen affinity purified polyclonal antibody (Catalog # A01427) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSD at approximately 130KD. The expected band size for PSD is at 109KD.
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