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ProductsBack/PSD antibody [C1C3]

PSD antibody [C1C3]

GTX118290
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Rat
TargetPSD
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Overview

  • Supplier
    GeneTex
  • Product Name
    PSD antibody [C1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.57 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5662
  • Target name
    PSD
  • Target description
    pleckstrin and Sec7 domain containing
  • Target synonyms
    EFA6, EFA6A, PSD1, TYL, PH and SEC7 domain-containing protein 1, exchange factor for ADP-ribosylation factor guanine nucleotide factor 6, exchange factor for ARF6 A, pleckstrin homology and SEC7 domain-containing protein 1, pleckstrin-Sec7 domains-containing
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDA5PKW4
  • Protein Name
    PH and SEC7 domain-containing protein 1
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of PSD using anti-PSD antibody (A01427). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSD antigen affinity purified polyclonal antibody (Catalog # A01427) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSD at approximately 130KD. The expected band size for PSD is at 109KD.
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