Anti-PSMA [7F12]

Absolute Antibody

Anti-PSMA [7F12]

7

This antibody binds a conformational epitope on the human PSMA. This antibody can bind native PSMA protein but were unreactive with any fusion protein containing a portion of the PSMA sequence. Heat denaturation of PSMA also results in loss of binding.

This antibody was capable of detecting membrane fractions from human prostatic adenocarcinoma LNCaP and PC3 cells in a solid phase ELISA. This antibody was used for immunoprecipitation of PSMA from LNCaP cell detergent lysates. Antibody binding competition assays were done using flow cytometry and it revealed that 7F12 binds an epitope closed to another murine anti-PSMA antibody 3C6. This antibody was shown to mediated antibody dependent cellular cytotoxicity in human prostatic adenocarcinoma LNCaP cell targets with PBMCs from two donors (WO2003064606). This antibody was also used in the treatment of in vivo tumor xenografts with a combination of Taxotere® (US20090297438).

ImmunoPrecipitation, ELISA

ELISA; IP;

Research Use Only

Monoclonal

7F12

FOLH1

folate hydrolase 1

cell growth-inhibiting gene 27 protein; FGCP; FOLH; folylpoly-gamma-glutamate carboxypeptidase; GCP2; GCPII; glutamate carboxylase II; glutamate carboxypeptidase 2; glutamate carboxypeptidase II; membrane glutamate carboxypeptidase; mGCP; NAALAD1; NAALADase I; N-acetylated alpha-linked acidic dipeptidase 1; N-acetylated-alpha-linked acidic dipeptidase I; prostate specific membrane antigen variant F; PSM; PSMA; pteroylpoly-gamma-glutamate carboxypeptidase

Mouse

IgG1

Glutamate carboxypeptidase 2

Human

Human

The original antibody was generated by immunizing mice with cell membranes and purified PSMA protein isolated from LNCaP cells.

-20°C,2°C to 8°C

12352203