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Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.

Anti-PSMA2 Antibody

HPA008188
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPSMA2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PSMA2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5683
  • Target name
    PSMA2
  • Target description
    proteasome 20S subunit alpha 2
  • Target synonyms
    HC3, MU, PMSA2, PSC2, proteasome subunit alpha type-2, alpha-2, macropain subunit C3, multicatalytic endopeptidase complex subunit C3, proteasome (prosome, macropain) subunit, alpha type, 2, proteasome component C3, proteasome subunit HC3, proteasome subunit alpha 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25787
  • Protein Name
    Proteasome subunit alpha type-2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PSMA2 using anti-PSMA2 antibody (PB10088). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat testis tissue lysates, Lane 2: MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMA2 antigen affinity purified polyclonal antibody (Catalog # PB10088) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMA2 at approximately 26 kDa. The expected band size for PSMA2 is at 26 kDa.
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