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Immunohistochemical staining of human placenta shows moderate to strong nuclear and cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows moderate to strong nuclear and cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human placenta shows moderate to strong nuclear and cytoplasmic positivity in trophoblastic cells.

Anti-PSMB2 Antibody

HPA026322
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPSMB2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PSMB2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5690
  • Target name
    PSMB2
  • Target description
    proteasome 20S subunit beta 2
  • Target synonyms
    HC7-I; macropain subunit C7-I; multicatalytic endopeptidase complex subunit C7-1; multicatalytic endopeptidase complex subunit C7-I; proteasome (prosome, macropain) subunit, beta type, 2; proteasome beta 2 subunit; proteasome component C7-I; proteasome subunit beta 2; proteasome subunit beta type-2; proteasome subunit beta4; proteasome subunit, beta type, 2; testicular tissue protein Li 152
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49721
  • Protein Name
    Proteasome subunit beta type-2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PSMB2 using anti-PSMB2 antibody (A07697-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMB2 antigen affinity purified polyclonal antibody (Catalog # A07697-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMB2 at approximately 23 kDa. The expected band size for PSMB2 is at 23 kDa.
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