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Western blot All lanes: proteasome subunit beta type-2 antibody at 2ug/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 23 kDa Observed band size: 23 kDa
Western blot All lanes: proteasome subunit beta type-2 antibody at 2ug/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 23 kDa Observed band size: 23 kDa
Western blot All lanes: proteasome subunit beta type-2 antibody at 2ug/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 23 kDa Observed band size: 23 kDa

PSMB2 Antibody

CSB-PA01385A0RB
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPSMB2
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Overview

  • Supplier
    Cusabio
  • Product Name
    PSMB2 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5690
  • Target name
    PSMB2
  • Target description
    proteasome 20S subunit beta 2
  • Target synonyms
    HC7-I; macropain subunit C7-I; multicatalytic endopeptidase complex subunit C7-1; multicatalytic endopeptidase complex subunit C7-I; proteasome (prosome, macropain) subunit, beta type, 2; proteasome beta 2 subunit; proteasome component C7-I; proteasome subunit beta 2; proteasome subunit beta type-2; proteasome subunit beta4; proteasome subunit, beta type, 2; testicular tissue protein Li 152
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49721
  • Protein Name
    Proteasome subunit beta type-2
  • Scientific Description
    The proteasome is a multicatalytic proteinase complex with a highly ordered ring shaped 20S core structure. The core structure is composed of 4 rings of 28 non identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMB2 is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit.The proteasome is a multicatalytic proteinase complex with a highly ordered ring shaped 20S core structure. The core structure is composed of 4 rings of 28 non identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin dependent process in a non lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B type family, also known as the T1B family, that is a 20S core beta subunit. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PSMB2 using anti-PSMB2 antibody (A07697-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMB2 antigen affinity purified polyclonal antibody (Catalog # A07697-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMB2 at approximately 23 kDa. The expected band size for PSMB2 is at 23 kDa.
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