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Immunohistochemical staining of human cerebellum shows strong nuclear and cytoplasmic positivity in purkinje cells.
Immunohistochemical staining of human cerebellum shows strong nuclear and cytoplasmic positivity in purkinje cells.
Immunohistochemical staining of human cerebellum shows strong nuclear and cytoplasmic positivity in purkinje cells.

Anti-PSMC4 Antibody

HPA005471
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetPSMC4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PSMC4
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5704
  • Target name
    PSMC4
  • Target description
    proteasome 26S subunit, ATPase 4
  • Target synonyms
    26S protease regulatory subunit 6B; 26S proteasome AAA-ATPase subunit RPT3; 26S proteasome regulatory subunit 6B; MB67-interacting protein; MIP224; protease 26S subunit 6; proteasome (prosome, macropain) 26S subunit, ATPase, 4; RPT3; S6; Tat-binding protein 7; TBP7; TBP-7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP43686
  • Protein Name
    26S proteasome regulatory subunit 6B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Western Blot Positive WB detected in: Mouse liver tissue All lanes: PSMC4 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 48, 44 kDa Observed band size: 48 kDa
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Figure 1. Western blot analysis of PSMC4 using anti-PSMC4 antibody (A06099-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human U251 whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSMC4 antigen affinity purified polyclonal antibody (Catalog # A06099-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSMC4 at approximately 50 kDa. The expected band size for PSMC4 is at 43-50 kDa.
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