
Figure 1. Western blot analysis of PSME2 using anti-PSME2 antibody (A07053-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: rat NRK whole cell lysates, Lane 7: rat C6 whole cell lyastes, Lane 8: mouse SP2/0 whole cell lysates, Lane 9: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSME2 antigen affinity purified polyclonal antibody (Catalog # A07053-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSME2 at approximately 32KD. The expected band size for PSME2 is at 27KD.
Anti-PSME2 Antibody Picoband(r)
A07053-1-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetPSME2
Overview
- SupplierBoster Bio
- Product NameAnti-PSME2 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID5721
- Target namePSME2
- Target descriptionproteasome activator subunit 2
- Target synonyms11S regulator complex beta subunit; 11S regulator complex subunit beta; activator of multicatalytic protease subunit 2; cell migration-inducing protein 22; MCP activator, 31-kD subunit; PA28B; PA28beta; proteasome (prosome, macropain) activator subunit 2 (PA28 beta); proteasome activator 28 subunit beta; proteasome activator 28-beta; proteasome activator complex subunit 2; proteasome activator hPA28 subunit beta; REGbeta; REG-beta
- HostRabbit
- IsotypeIgG
- Protein IDQ9UL46
- Protein NameProteasome activator complex subunit 2
- Scientific DescriptionBoster Bio Anti-PSME2 Antibody Picoband® catalog # A07053-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203