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IHC-P analysis of mouse hepatocarcinoma using GTX81770 PSME2 antibody, C-term.
IHC-P analysis of mouse hepatocarcinoma using GTX81770 PSME2 antibody, C-term.
IHC-P analysis of mouse hepatocarcinoma using GTX81770 PSME2 antibody, C-term.

PSME2 antibody, C-term

GTX81770
GeneTex
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPSME2
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Overview

  • Supplier
    GeneTex
  • Product Name
    PSME2 antibody, C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. IHC-P: 1:50-1:100. FACS: 1:10-1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5721
  • Target name
    PSME2
  • Target description
    proteasome activator subunit 2
  • Target synonyms
    11S regulator complex beta subunit; 11S regulator complex subunit beta; activator of multicatalytic protease subunit 2; cell migration-inducing protein 22; MCP activator, 31-kD subunit; PA28B; PA28beta; proteasome (prosome, macropain) activator subunit 2 (PA28 beta); proteasome activator 28 subunit beta; proteasome activator 28-beta; proteasome activator complex subunit 2; proteasome activator hPA28 subunit beta; REGbeta; REG-beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UL46
  • Protein Name
    Proteasome activator complex subunit 2
  • Scientific Description
    The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the beta subunit of the 11S regulator, one of the two 11S subunits that is induced by gamma-interferon. Three beta and three alpha subunits combine to form a heterohexameric ring. Six pseudogenes have been identified on chromosomes 4, 5, 8, 10 and 13. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PSME2 using anti-PSME2 antibody (A07053-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: rat NRK whole cell lysates, Lane 7: rat C6 whole cell lyastes, Lane 8: mouse SP2/0 whole cell lysates, Lane 9: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PSME2 antigen affinity purified polyclonal antibody (Catalog # A07053-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PSME2 at approximately 32KD. The expected band size for PSME2 is at 27KD.
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