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Immunohistochemical staining of human cerebellum shows moderate positivity in neuronal processes in molecular layer.
Immunohistochemical staining of human cerebellum shows moderate positivity in neuronal processes in molecular layer.
Immunohistochemical staining of human cerebellum shows moderate positivity in neuronal processes in molecular layer.

Anti-PTH2R Antibody

HPA010655
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPTH2R
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PTH2R Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5746
  • Target name
    PTH2R
  • Target description
    parathyroid hormone 2 receptor
  • Target synonyms
    PTHR2, parathyroid hormone 2 receptor, PTH2 receptor, parathyroid hormone receptor 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49190
  • Protein Name
    Parathyroid hormone 2 receptor
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PTH2R using anti-PTH2R antibody (A10343-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTH2R antigen affinity purified polyclonal antibody (Catalog # A10343-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTH2R at approximately 75 kDa. The expected band size for PTH2R is at 62 kDa.
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