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PTHR2 / PTH2R Antibody (HRP)

LS-C317886
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPTH2R
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PTHR2 / PTH2R Antibody (HRP)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    HRP
  • Estimated Purity
    ...
  • Gene ID5746
  • Target name
    PTH2R
  • Target description
    parathyroid hormone 2 receptor
  • Target synonyms
    PTHR2, parathyroid hormone 2 receptor, PTH2 receptor, parathyroid hormone receptor 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PTH2R using anti-PTH2R antibody (A10343-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTH2R antigen affinity purified polyclonal antibody (Catalog # A10343-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTH2R at approximately 75 kDa. The expected band size for PTH2R is at 62 kDa.
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