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Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.

Anti-PTK2 Antibody

HPA001842
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPTK2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PTK2
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5747
  • Target name
    PTK2
  • Target description
    protein tyrosine kinase 2
  • Target synonyms
    FADK, FADK 1, FAK, FAK1, FRNK, PPP1R71, p125FAK, pp125FAK, focal adhesion kinase 1, FAK-related non-kinase polypeptide, PTK2 protein tyrosine kinase 2, focal adhesion kinase-related nonkinase, protein phosphatase 1 regulatory subunit 71
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ05397
  • Protein Name
    Focal adhesion kinase 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of FAK/PTK2 using anti-FAK/PTK2 antibody (PB9674). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates. Lane 3: human K562 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat C6 whole cell lysates, Lane 9: mouse lung tissue lysates, Lane 10: mouse brain tissue lysates, Lane 11: mouse RAW264.7 whole cell lysates, Lane 12: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAK/PTK2 antigen affinity purified polyclonal antibody (Catalog # PB9674) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAK/PTK2 at approximately 125 kDa. The expected band size for FAK/PTK2 is at 119 kDa.
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