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Anti-PTN (N-term) Antibody

102-27382
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPTN
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-PTN (N-term) Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB14475
  • Gene ID5764
  • Target name
    PTN
  • Target description
    pleiotrophin
  • Target synonyms
    HARP, HB-GAM, HBBM, HBGF-8, HBGF8, HBNF, HBNF-1, NEGF1, OSF-1, pleiotrophin, heparin affin regulatory protein, heparin-binding brain mitogen, heparin-binding growth factor 8, heparin-binding growth-associated molecule, heparin-binding neurite outgrowth promoting factor, heparin-binding neurite outgrowth-promoting factor 1, osteoblast-specific factor 1, pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1)
  • Host
    Rabbit
  • Protein IDP21246
  • Protein Name
    Pleiotrophin
  • Scientific Description
    Rabbit Anti-PTN (N-term) Antibody, 400 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Pleiotrophin using anti-Pleiotrophin antibody (A01368-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pleiotrophin antigen affinity purified polyclonal antibody (Catalog # A01368-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pleiotrophin at approximately 19KD. The expected band size for Pleiotrophin is at 19KD.
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