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ProductsBack/PTN Polyclonal Antibody, AbBy Fluor-350 Conjugated

PTN Polyclonal Antibody, AbBy Fluor-350 Conjugated

BS-21928R-BF350
Bioss Antibodies
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPTN
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    PTN Polyclonal Antibody, AbBy Fluor-350 Conjugated
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Other Conjugate
  • Gene ID5764
  • Target name
    PTN
  • Target description
    pleiotrophin
  • Target synonyms
    HARP; HBBM; HB-GAM; HBGF8; HBGF-8; HBNF; HBNF-1; heparin affin regulatory protein; heparin-binding brain mitogen; heparin-binding growth factor 8; heparin-binding growth-associated molecule; heparin-binding neurite outgrowth promoting factor; heparin-binding neurite outgrowth-promoting factor 1; NEGF1; OSF-1; osteoblast-specific factor 1; pleiotrophin; pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP21246
  • Protein Name
    Pleiotrophin
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Pleiotrophin using anti-Pleiotrophin antibody (A01368-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Pleiotrophin antigen affinity purified polyclonal antibody (Catalog # A01368-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Pleiotrophin at approximately 19KD. The expected band size for Pleiotrophin is at 19KD.
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