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Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in subset of germinal center cells.
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in subset of germinal center cells.
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in subset of germinal center cells.

Anti-PTPRJ Antibody

HPA006026
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPTPRJ
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PTPRJ
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5795
  • Target name
    PTPRJ
  • Target description
    protein tyrosine phosphatase receptor type J
  • Target synonyms
    CD148, DEP1, HPTP eta, HPTPeta, R-PTP-ETA, R-PTP-J, SCC1, THC10, receptor-type tyrosine-protein phosphatase eta, CD148 antigen, density-enhanced phosphatase 1, human density enhanced phosphatase-1, protein tyrosine phosphatase, receptor type, J polypeptide, protein-tyrosine phosphatase eta, susceptibility to colon cancer 1, mouse, homolog of
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12913
  • Protein Name
    Receptor-type tyrosine-protein phosphatase eta
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of DEP1/PTPRJ using anti-DEP1/PTPRJ antibody (A02319-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DEP1/PTPRJ antigen affinity purified polyclonal antibody (Catalog # A02319-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DEP1/PTPRJ at approximately 240 kDa. The expected band size for DEP1/PTPRJ is at 146 kDa.
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