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Western Blot analysis of HeLa, 293 cells using CD148 Polyclonal Antibody.
Western Blot analysis of HeLa, 293 cells using CD148 Polyclonal Antibody.
Western Blot analysis of HeLa, 293 cells using CD148 Polyclonal Antibody.

PTPRJ Antibody

CSB-PA005636
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetPTPRJ
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Overview

  • Supplier
    Cusabio
  • Product Name
    PTPRJ Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5795
  • Target name
    PTPRJ
  • Target description
    protein tyrosine phosphatase receptor type J
  • Target synonyms
    CD148; CD148 antigen; density-enhanced phosphatase 1; DEP1; DEP-1; HPTP eta; HPTPeta; human density enhanced phosphatase-1; protein tyrosine phosphatase, receptor type, J polypeptide; protein-tyrosine phosphatase eta; receptor-type tyrosine-protein phosphatase eta; R-PTP-ETA; R-PTP-J; SCC1; susceptibility to colon cancer 1, mouse, homolog of
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ12913
  • Protein Name
    Receptor-type tyrosine-protein phosphatase eta
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of DEP1/PTPRJ using anti-DEP1/PTPRJ antibody (A02319-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DEP1/PTPRJ antigen affinity purified polyclonal antibody (Catalog # A02319-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DEP1/PTPRJ at approximately 240 kDa. The expected band size for DEP1/PTPRJ is at 146 kDa.
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