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Immunohistochemical staining of human breast shows strong cytoplasmic and membranous positivity in myoepithelial cells.
Immunohistochemical staining of human breast shows strong cytoplasmic and membranous positivity in myoepithelial cells.
Immunohistochemical staining of human breast shows strong cytoplasmic and membranous positivity in myoepithelial cells.

Anti-PTRF Antibody

HPA049838
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCAVIN1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PTRF Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID284119
  • Target name
    CAVIN1
  • Target description
    caveolae associated protein 1
  • Target synonyms
    CAVIN, CGL4, FKSG13, PTRF, cavin-1, caveolae-associated protein 1, RNA polymerase I and transcript release factor, TTF-I interacting peptide 12, congenital generalized lipodystrophy 4, polymerase I and transcript release factor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6NZI2
  • Protein Name
    Caveolae-associated protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PTRF/CAVIN1 using anti-PTRF/CAVIN1 antibody (A31732-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse heart tissue lysates, Lane 6: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTRF/CAVIN1 antigen affinity purified polyclonal antibody (Catalog # A31732-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTRF/CAVIN1 at approximately 50-55 kDa. The expected band size for PTRF/CAVIN1 is at 43 kDa.
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