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Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.

Anti-PUDP Antibody

HPA072672
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPUDP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PUDP Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8226
  • Target name
    PUDP
  • Target description
    pseudouridine 5'-phosphatase
  • Target synonyms
    DXF68S1E, FAM16AX, GS1, HDHD1, HDHD1A, pseudouridine-5'-phosphatase, 5'-PsiMPase, family with sequence similarity 16, member A, X-linked, haloacid dehalogenase-like hydrolase domain containing 1, haloacid dehalogenase-like hydrolase domain containing 1A, haloacid dehalogenase-like hydrolase domain-containing protein 1, haloacid dehalogenase-like hydrolase domain-containing protein 1A, pseudouridine-5'-monophosphatase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ08623
  • Protein Name
    Pseudouridine-5'-phosphatase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of PUDP using anti-PUDP antibody (A13748-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PUDP antigen affinity purified polyclonal antibody (Catalog # A13748-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PUDP at approximately 25 kDa. The expected band size for PUDP is at 25 kDa.
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