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ProductsBack/HDHD1 Antibody (clone 4F12)

HDHD1 Antibody (clone 4F12)

LS-C198537
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetPUDP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HDHD1 Antibody (clone 4F12)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB Sandwich ELISA: Recombinant protein. Western Blot: Transfected lysate, Recombinant protein.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4F12
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8226
  • Target name
    PUDP
  • Target description
    pseudouridine 5'-phosphatase
  • Target synonyms
    DXF68S1E, FAM16AX, GS1, HDHD1, HDHD1A, pseudouridine-5'-phosphatase, 5'-PsiMPase, family with sequence similarity 16, member A, X-linked, haloacid dehalogenase-like hydrolase domain containing 1, haloacid dehalogenase-like hydrolase domain containing 1A, haloacid dehalogenase-like hydrolase domain-containing protein 1, haloacid dehalogenase-like hydrolase domain-containing protein 1A, pseudouridine-5'-monophosphatase
  • Host
    Mouse
  • Isotype
    IgG2a
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PUDP using anti-PUDP antibody (A13748-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PUDP antigen affinity purified polyclonal antibody (Catalog # A13748-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PUDP at approximately 25 kDa. The expected band size for PUDP is at 25 kDa.
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