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Immunohistochemical staining of human small intestine shows strong granular positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong granular positivity in glandular cells.
Immunohistochemical staining of human small intestine shows strong granular positivity in glandular cells.

Anti-PYCRL Antibody

HPA069706
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPYCR3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-PYCRL Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID65263
  • Target name
    PYCR3
  • Target description
    pyrroline-5-carboxylate reductase 3
  • Target synonyms
    PYCRL, pyrroline-5-carboxylate reductase 3, P5C reductase 3, P5CR 3, pyrroline-5-carboxylate reductase-like
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ53H96
  • Protein Name
    Pyrroline-5-carboxylate reductase 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PYCRL/PYCR3 using anti-PYCRL/PYCR3 antibody (A32428-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse small intestine tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYCRL/PYCR3 antigen affinity purified polyclonal antibody (A32428-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PYCRL/PYCR3 at approximately 29 kDa. The expected band size for PYCRL/PYCR3 is at 29 kDa.
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