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Anti-PYGL Antibody

144-06710
RayBiotech
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetPYGL
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-PYGL Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5836
  • Target name
    PYGL
  • Target description
    glycogen phosphorylase L
  • Target synonyms
    GSD6, glycogen phosphorylase, liver form, phosphorylase, glycogen, liver
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06737
  • Protein Name
    Glycogen phosphorylase, liver form
  • Scientific Description
    PYGL Polyclonal Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PYGL using anti-PYGL antibody (A06317-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYGL antigen affinity purified polyclonal antibody (Catalog # A06317-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PYGL at approximately 97 kDa. The expected band size for PYGL is at 97 kDa.
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