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Western blot All lanes: PYGL antibody at 2microg/ml Lane 1: 293T whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 98, 94 kDa Observed band size: 98 kDa
Western blot All lanes: PYGL antibody at 2microg/ml Lane 1: 293T whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 98, 94 kDa Observed band size: 98 kDa
Western blot All lanes: PYGL antibody at 2microg/ml Lane 1: 293T whole cell lysate Lane 2: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 98, 94 kDa Observed band size: 98 kDa

PYGL Antibody

CSB-PA019122EA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetPYGL
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Overview

  • Supplier
    Cusabio
  • Product Name
    PYGL Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5836
  • Target name
    PYGL
  • Target description
    glycogen phosphorylase L
  • Target synonyms
    GSD6, glycogen phosphorylase, liver form, phosphorylase, glycogen, liver
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP06737
  • Protein Name
    Glycogen phosphorylase, liver form
  • Scientific Description
    Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of PYGL using anti-PYGL antibody (A06317-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYGL antigen affinity purified polyclonal antibody (Catalog # A06317-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PYGL at approximately 97 kDa. The expected band size for PYGL is at 97 kDa.
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